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| Overview |
The CD271 (LNGFR) antibody can be used to identify and enumerate CD271 (LNGFR)+ cells by flow cytometry or fluorescence microscopy. PE- or APC-conjugated antibodies is recommended for staining cells, e.g. MSCs, neural cells, etc.
The CD271 (LNGFR) antibody can be used to monitor the transfection efficiency of cells transfected with pMACS LNGFR or pMACS LNGFR-IRES plasmids before labeling with MACSelect LNGFR MicroBeads when using the MACSelect™ LNGFR Transfected Cell Selection System. |
| Details |
Background information
Clone ME20.4-1.H4 recognizes the CD271 antigen, also known as LNGFR (low-affinity nerve growth factor receptor) or p75 NTR (neurotrophin receptor). CD271 belongs to the low-affinity neurotrophin receptors and the tumor necrosis factor receptor superfamily. Initially the human CD271 (LNGFR) was described to be expressed on cells of the central and peripheral nervous system, and was suggested to be involved in the development, survival, and differentiation of cells.1
CD271 (LNGFR) is expressed on
- Mesenchymal stem/stromal cells (MSCs)2,3,4
- Follicular dendritic cells5
- Mesenchymal cells involved in mesenchymal-epithelial interactions6
- Autonomic and sensory Neurons7
- Oligodendrocytes8
- Astrocytes9
- Schwann cells10,11
- Neural crest stem cells12
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| Cross-reactivity |
| The CD271 antibody shows cross-reactivity with monkey, goat, dog, pig and sheep13. |
| Clone | Isotype |
| ME20.4-1.H4 | Mouse IgG1 |
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| Figure 1 |
| Human bone marrow cells were stained with CD271 (LNGFR)-APC and CD45-FITC and analyzed by flow cytometry. |
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| Figure 2 |
| Jurkat cells were transfected with the human LNGFR gene and mixed with untransfected cells. Mixed cells were stained with fluorochrome-conjugated CD271 (LNGFR) antibodies conjugated to PE (A) and APC (B) and then analyzed by flow cytometry. |
| A |
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| B |
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| Products |
| CD271 (LNGFR)-FITC, human |
- for 100 tests (1)
Download datasheet
130-091-917
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| CD271 (LNGFR)-PE, human |
- for 100 tests (1)
Download datasheet
130-091-885
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| CD271 (LNGFR)-APC, human |
- for 100 tests (1)
Download datasheet
130-091-884
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| CD271 (LNGFR)-Biotin, human |
- for 100 tests (2)
Download datasheet
130-091-883
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 μL. |
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| References |
| 1. Thomson et al. (1988) Exp. Cell Res. 174: 533–539. |
| 2. Caneva et al. (1995) Blood Cells Mol. Dis. 21: 73–85. |
| 3. Quirici et al. (2002) Exp. Hematol. 30: 783–791. |
| 4. Jones et al. (2002) Arthritis & Rheumatism 46: 3349–3360. |
| 5. Pezzati et al. (1992) Immunology 76: 485–490. |
| 6. Huber and Chao (1995) Dev. Biol. 167: 227–238. |
| 7. Kashiba et al. (1995) Mol. Brain Res. 30: 158–164. |
| 8. Casaccia-Bonnefil, P. et al. (1996) Nature 383: 716–719. |
| 9. Rudge et al. (1994) Eur. J. Neurosci. 6: 693–705. |
| 10. DiStefano and Johnson (1988) Proc. Natl. Acad. Sci. USA 85: 270–274. |
| 11. Vroemen and Weidner (2003) J Neuroscience Methods 124: 135–143. |
| 12. Chalazonitis, A. et al. (1998) Dev. Biol. 204 (2): 385-406. |
| 13. Rozemuller, H. et al. (2010) Stem Cells Dev. 19: 1911-1921. |
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