CD11b (human and mouse) ( #130-097-299 #130-097-336 #130-081-201 #130-091-240 #130-091-241 #130-096-834 )

CD11b (human and mouse)

Overview

The CD11b antibody can be used to identify and enumerate CD11b⁺ cells by flow cytometry or fluorescence microscopy. The monoclonal M1/70.15.11.5 antibody recognizes the human, mouse, and non-human primate CD11b antigen.

Details

Background information
CD11b (Mac-1 α; integrin αM chain) is part of the CD11b/CD18 heterodimer (Mac-1 α, Mß2 integrin), also known as the C3 complement receptor. It functions as a receptor for complement (C3bi), fibrinogen, or clotting factor X.
In humans, CD11b is strongly expressed on myeloid cells and weakly expressed on NK cells and some activated lymphocytes as well as on microglia in the brain.
In mice, the CD11b antigen is expressed on monocytes/macrophages and microglia. To a lower extent it is expressed on granulocytes, NK cells, CD5⁺ B-1 cells, and subsets of dendritic cells.

Cross-reactivity

This antibody has been tested to react with

Rhesus monkey (Macaca mulatta)
Cynomolgus monkey (Macaca fascicularis)

Clone	Isotype
M1/70.15.11.5	Rat IgG2b

Figure 1

Human PBMCs were stained with CD11b antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), VioGreen (E) or APC-Vio770 (F), and analyzed by flow cytometry.

Figure 2

Mouse splenocytes were stained with CD11b-PE and analyzed by flow cytometry.

Figure 3

Rhesus monkey PBMCs were stained with CD11b-FITC and analyzed by flow cytometry.

Figure 4

Cynomolgus monkey peripheral blood cells were stained with CD11b-APC and analyzed by flow cytometry. Data were kindly provided by the California National Primate Research Center, Davis, CA, USA.