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| Overview |
| The clone ACSA-1 (astrocyte cell surface antigen-1) recognizes an extracellular epitope of the astrocyte specific glutamate transporter GLAST and can be used for identification of GLAST+ astrocytes by flow cytometry or fluorescence microscopy. |
| Details |
Background information
The antibody ACSA-1 (astrocyte cell surface antigen-1) is specific for an extracellular epitope of the astrocyte specific transmembrane glycoprotein GLAST in the central nervous system (CNS).1,2 Besides GLT-1, GLAST is the most abundant glutamate transporter and is predominantly expressed by astrocytes in the developing and neonatal mammalian CNS. Also radial glia, which belong to the astrocyte lineage and play important roles in development, are known to express GLAST. Postnatally, radial glia only persist in a few regions, such as Bermann glia in the cerebellum, Müller glia in the retina, and radial glia in the dentate gyrus of the adult hippocampus.3,4 |
| Clone | Isotype |
| ACSA-1 | Mouse IgG2a |
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| Figure 1 |
Mouse brain tissue postnatal day 7 was dissociated using the Neural Tissue Dissociation Kit (T) and the gentleMACS™ Dissociator. Brain cells were stained using Anti-GLAST (ACSA‑1) antibodies conjugated to PE (A) or APC (B) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with Anti‑GLAST (ACSA-1)-Biotin (C) were stained with Anti‑Biotin‑APC.
Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
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| References |
| 1. Storck et al. (1992) Proc. Natl. Acad. Sci. USA 8 (22): 10955–10959. |
| 2. Wahle et al. (1996) J. Cell Biol. 135(6): 1867–1877. |
| 3. Kimelberg et al. (2004) Neurochem. Int. 45(2-3): 191–202. |
| 4. Kriegstein et al. (2003) Glia 43: 37–43. |
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