CD11b (Microglia) MicroBeads ( #130-093-634 )

CD11b (Microglia) MicroBeads

Overview

CD11b (Microglia) MicroBeads have been developed for the positive selection of mouse and human CD11b⁺ microglia.

Details

Background information
Microglia, often called brain macrophages, are the resident immune effector cells in the central nervous system (CNS). In addition, activated microglia serve as the major antigen-presenting cells in the CNS.¹ They are morphologically, immunophenotypically, and functionally related to cells of the monocyte/macrophage lineage.² For the isolation of microglia from myelin-containing tissue samples, the use of Myelin Removal Beads II is highly recommended.

Downstream applications
CD11b (Microglia) MicroBeads have been used to isolate CD11b⁺ cells from single-cell suspensions of brain tissue^3–6.

Columns

MS, LS, XS, or autoMACS® Columns.

Further information

Semi-automated tissue dissociation and preserved epitope integrity optimize immunomagnetic sorting of neural cells
[PDF; 310,8 KB]
Neuroscience research brochure
[PDF; 1,7 MB]

Figure 1

Whole-brain tissue from P1 mice was first dissociated to single cells using the Neural Tissue Dissociation Kit (T) and then microglia were isolated using CD11b (Microglia) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are fluorescently stained with CD11b-APC and CD45-FITC.

A: Before separation

B: Microglia CD11b⁺ fraction

$Microglia CD11b<sup>+</sup> fraction$