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| Astrocyte Isolation Starter Kits |
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| Overview |
| The Astrocyte Isolation Starter Kit offers the complete solution for the dissociation and isolation of GLAST+ astrocytes from rats or mice younger than postnatal day 7. A list of all products included within the Astrocyte Isolation Starter Kit is provided in the table within figure 1. |
| Details |
Background information
The Astrocyte Isolation Starter Kit includes the Neural Tissue Dissociation Kit (T), which guarantees conservation of the GLAST epitope for the isolation step. The Anti-GLAST (ACSA-1) MicroBead Kit isolates rat or mouse astrocytes which are positive for GLAST.
The antibody ACSA-1 (astrocyte cell surface antigen-1) recognizes an extracellular epitope of the astrocyte specific transmembrane glycoprotein GLAST.1,2 Besides GLT-1, GLAST is the most abundant glutamate transporter and is predominantly expressed by astrocytes in the developing and neonatal mammalian CNS. Also radial glia, which belong to the astrocyte lineage and play important roles in development, are known to express GLAST. |
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| Figure 1 |
| Included products of Astrocyte Isolation Starter Kit |
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| Products |
| Astrocyte Isolation Starter Kit, rat <P7 |
Components
- Neural Tissue Dissociation Kit (T)
- MACS® MultiStand
- MiniMACS™ Separator
- MS Columns
- Anti-GLAST (ACSA-1) MicroBead Kit, human, mouse and rat
Download datasheet
130-096-052
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| Astrocyte Isolation Starter Kit, mouse <P7 |
Components
- Neural Tissue Dissociation Kit (T)
- MACS® MultiStand
- MiniMACS™ Separator
- MS Columns
- Anti-GLAST (ACSA-1) MicroBead Kit, human, mouse and rat
Download datasheet
130-096-054
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| References |
| 1. Storck et al. (1992) Proc. Natl. Acad. Sci. USA 8 (22):10955–10959. |
| 2. Wahle et al. (1996) J. Cell Biol. 135(6): 1867–1877. |
| 3. Kimelberg et al. (2004) Neurochem. Int. 45(2-3): 191–202. |
| 4. Kriegstein et al. (2003) Glia 43: 37–43. |
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