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 - Groundbreaking innovation: isolate astrocytes in only one hour instead of two weeks
- Proven and easy: based on fast and powerful MACS Technology
- Reliable: excellent viability and purity of isolated astrocytes
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| Overview |
| The Anti-GLAST (ACSA-1) MicroBead Kit has been developed for the separation of astrocytes based on the expression of GLAST. |
| Details |
Background information
The antibody ACSA-1 (astrocyte cell surface antigen-1) is specific for an extracellular epitope of the astrocyte specific transmembrane glycoprotein GLAST in the central nervous system (CNS).1,2 Besides GLT-1, GLAST is the most abundant glutamate transporter and is predominantly expressed by astrocytes in the developing and neonatal mammalian CNS. Also radial glia, which belong to the astrocyte lineage and play important roles in development, are known to express GLAST. Postnatally, radial glia only persist in a few regions, such as Bermann glia in the cerebellum, Müller glia in the retina, and radial glia in the dentate gyrus of the adult hippocampus.3,4 |
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| Figure 1 |
| GLAST+ cells (positive fraction) were isolated from day 7 postnatal mouse brain tissue using the Neural Tissue Dissociation Kit (T), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-GLAST (ACSA-1) MicroBead Kit, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Anti-GLAST (ACSA-1)-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
| Mouse forebrain cells before separation |
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| GLAST (ACSA-1) -positive cells |
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| GLAST (ACSA-1)-negative cells |
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| Products |
| Anti-GLAST (ACSA-1) MicroBead Kit, human, mouse and rat |
Download datasheet
130-095-826
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| References |
| 1. Storck et al. (1992) Proc. Natl. Acad. Sci. USA 8 (22):10955–10959. |
| 2. Wahle et al. (1996) J. Cell Biol. 135(6): 1867–1877. |
| 3. Kimelberg et al. (2004) Neurochem. Int. 45(2-3): 191–202. |
| 4. Kriegstein et al. (2003) Glia 43: 37–43. |
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