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| Neural Tissue Dissociation Kits |
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| Overview |
The Neural Tissue Dissociation Kits have been developed for the gentle and efficient generation of single-cell suspensions from neural tissues.
Depending on the application the kits can be used with manual mechanical dissociation or with automated dissociation, namely the gentleMACS™ Dissociator and the gentleMACS Octo Dissociator.
The Neural Tissue Dissociation Kit - Postnatal Neurons has been optimized for the special needs of sensitive postnatal neurons during dissociation. |
| Details |
Background information
In less than an hour, the gentle two-step enzymatic dissociation procedure efficiently yields high numbers of viable cells. The Neural Tissue Dissociation Kit (P) contains papain, and the Neural Tissue Dissociation Kit (T) contains trypsin.
Some antigen epitopes are damaged by papain, others by trypsin. The kit based on papain is best suited for use with Anti-Prominin-1 MicroBeads, CD11b (Microglia) MicroBeads and Anti-A2B5 MicroBeads, whereas, for example, Anti-GLAST (ACSA-1) MicroBead Kit and Anti-PSA-NCAM MicroBeads require the use of the trypsin-based kit.
Please see figure 1 for further information with regard to epitope sensitivity and kit selection. |
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| Figure 1 |
Antigen compatibility of Dissociation Kits.
For many applications, including MACS Cell Separation, it is crucial to conserve the integrity of particular cell surface epitopes during tissue dissociation. The table shows which kits conserve which specific epitopes. Epitope sensitivities have been tested using Miltenyi Biotec's antibodies if available. |
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| Products |
| Neural Tissue Dissociation Kit (P) |
| For research use only |
- for 50 preparations
Download datasheet
130-092-628
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| Neural Tissue Dissociation Kit (T) |
- for 50 preparations
Download datasheet
130-093-231
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| Neural Tissue Dissociation Kit – Postnatal Neurons |
- for 50 preparations
Download datasheet
130-094-802
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| References |
| 1. Környei et al. (2007) FASEB J. 21: 2496–2509. |
| 2. Lee et al. (2008) J. Neurosci. 28: 8517-8528. |
| 3. Skog et al. (2008) Nat. Cell. Biol. 10 (12): 1470-1476. |
| 4. Szulwach et al. (2010) J. Cell Biol. 189: 127-141. |
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