Brain Tumor Dissociation Kits, human

Gentle and reliable dissociation
Reproducible results with automation
Quick procedure with high cell yield

Overview

The Brain Tumor Dissociation Kits have been developed for the gentle and effective generation of single-cell suspensions from human brain tumors, and can be used in combination with the gentleMACS™ Dissociator and the gentleMACS Octo Dissociator. In less than an hour, the gentle enzymatic dissociation procedure efficiently yields high numbers of viable cells. Dissociated cells are immediately ready for further in vitro or in vivo applications such as cell culture and expansion, flow cytometry, fluorescence microscopy, and molecular applications, or for cell separations using MACS® Technology .

Details

Background information
Human brain tumor samples can be effectively dissociated into single-cell suspensions using the Brain Tumor Dissociation Kit¹. The quality of starting material is important for any experiment, and a good single-cell suspension is a prerequisite for many downstream applications.

The Brain Tumor Dissociation Kits (T) and (P) are based on trypsin and papain respectively. Some antigen epitopes are degraded by papain, others by trypsin. The kit based on papain is best suited for use with CD133 MicroBeads, CD11b (Microglia) MicroBeads and Anti-A2B5 MicroBeads, whereas, for example, the Anti-GLAST (ACSA-1) MicroBead Kit requires the use of the trypsin-based kit.
Please see Figure 1 for further information with regard to epitope sensitivity and kit selection.

Further information

Standardized tissue dissociation and removal of myelin debris optimize isolation of cells from human brain tumor
[PDF; 1,2 MB]
Neuroscience research brochure
[PDF; 1,7 MB]

Figure 1

Antigen compatibility of Dissociation Kits.
For many applications, including MACS Cell Separation, it is crucial to conserve the integrity of particular cell surface epitopes during tissue dissociation. The table shows which kits conserve which specific epitopes. Epitope sensitivities have been tested using Miltenyi Biotec's antibodies if available.