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| Anti-MHC Class II MicroBeads |
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| Overview |
| Mouse Anti-MHC Class II MicroBeads are used for the positive selection or depletion of MHC class II+ cells from lymphoid and non-lymphoid tissue or from blood. |
| Details |
Background information
MHC class II is expressed on B cells, macrophages, monocytes, dendritic cells, thymic epithelial cells, and on subsets of hematopoietic progenitor cells. The MicroBeads bind to MHC class II haplotypes I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, which are expressed by the most common inbred mouse strains, e.g. C57BL/6, BALB/c, or 129/SvEv. MHC II haplotypes I-Af, I-Ak, and I-As are not recognized.
Downstream applications
Isolated MHC class II+ cells are suitable for studies on antigen presentation such as in T cell stimulation assays. Anti-MHC Class II MicroBeads were used for positive selection of Langerhans cells from epidermis1, for the isolation of untouched T cells from lymph nodes by depletion of MHC class II+ cells2, or in combination with other MicroBeads for isolation of untouched T cells from spleen3,4. |
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| Figure 1 |
| Isolation of untouched T cells from mouse lymph nodes by depletion of MHC class II expressing cells using Anti-MHC Class II MicroBeads, a MiniMACS™ Separator, and an MS Column. |
| Lymph node cells before separation |
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| Enriched T cells after depletion of MHC class II+ cells |
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| Favorites / Prices |
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| Details |
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| Products |
| Anti-MHC Class II MicroBeads, mouse |
- for 2×109 total cells
Download datasheet
130-052-401
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| References |
| 1. Sato et al. (2002) Eur. J. Immunol. 32: 3326–3335. |
| 2. Fruman et al. (1999) Science 283: 393–397. |
| 3. Lerner et al. (2000) J. Immunol. 164: 3996–4002. |
| 4. Foulds et al. (2002) J. Immunol. 168: 1528–1532. |
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