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| Plasmacytoid Dendritic Cell Isolation Kit II |
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| Description |
The Plasmacytoid Dendritic Cell Isolation Kit II is designed for the isolation of untouched plasmacytoid dendritic cells (PDCs) from single-cell suspensions of lymphoid organs.
Freshly isolated PDCs express low levels of MHC class II and costimulatory molecules and are poor stimulators of T helper cells. Upon stimulation with bacterial DNA containing particular unmethylated CpG motifs (CpG-DNA) or upon viral challenge, they produce large amounts of type I IFN and acquire antigen-presenting capacity.1,2 PDCs can be directly identified in lymphoid and non-lymphoid organs by the specific expression of mPDCA-1 (murine plasmacytoid dendritic cell antigen-1).3 Recently, mPDCA-1 has been identified as bone marrow stromal antigen 2 (BST2).4 Using the Plasmacytoid Dendritic Cell Isolation Kit II, non-PDCs are labeled with a cocktail of biotin-conjugated monoclonal antibodies and Anti-Biotin MicroBeads and depleted by retaining them on a MACS® Column in the magnetic field of a MACS Separator, while the highly enriched unlabeled PDCs pass through the column. |
| Applications |
| PDCs isolated by using the Plasmacytoid Dendritic Cell Isolation Kit II are suitable to analyze antigen uptake, antigen processing, T cell activation or T cell tolerance induction, cross-priming of cytotoxic T cells, and T helper cell polarization by PDCs. Isolated untouched PDCs can also be used for studies of PDC function where effects due to antibody-crosslinking of cell surface proteins should be avoided. |
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| Figure 1 |
| PDCs were isolated from a mouse spleen cell suspension by using the Plasmacytoid Dendritic Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with Anti-mPDCA-1-FITC and Anti-Ly-6C-APC. Cell debris and dead cells are excluded from the analysis based on scatter signals and PI fluorescence. |
| Spleen cells before separation |
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| Spleen cells depleted of plasmacytoid dendritic cells |
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| Isolated plasmacytoid dendritic cells |
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| Products |
| Plasmacytoid Dendritic Cell Isolation Kit II, mouse |
- for 2Ă—109 total cells
Download data sheet
130-092-786
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| MACS References |
| 1. Shortman, K. and Liu, Y. J. (2002) Mouse and human dendritic cell subtypes. Nat. Rev. Immunol. 2: 151–161. |
| 2. Nakano H. et al. (2001) CD11c+B220+Gr-1+ cells in mouse lymph nodes and spleen display characteristics of plasmacytoid dendritic cells. J. Exp. Med. 194: 1171–1178. |
| 3. MACS&more (2004) mPDCA-1: A presumably novel antigen exclusively expressed by murine plasmacytoid dendritic cells. Vol.8-1:13. |
| 4. Blasius, A. L. et al. (2006) Bone marrow stromal cell antigen 2 is a specific marker of type I IFN-producing cells in naive mouse, but a promiscuous cell surface antigen following IFN stimulation. J. Immunol. 177: 3260–3265. |
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