| Overview |
| Anti-mPDCA-1 MicroBeads have been developed for the enrichment of mouse plasmacytoid dendritic cells (PDCs) from lymphoid tissues. |
| Details |
Background information
The mouse plasmacytoid dendritic cell antigen-1 (mPDCA-1) is specifically expressed on mouse PDCs, a subset of CD11c+ dendritic cells detected at low frequency in all lymphoid tissues, peripheral blood, and some non-lymphoid tissues. In mouse spleen, bone marrow, and lymph nodes, mPDCA-1 is exclusively expressed on cells which are CD11clow, CD45R (B220)+, Ly-6Chigh, MHC Class IIlow, CD8alow/-, CD80low/-, CD86-, CD40-, CD11b-, CD90-, CD49b (DX5)-, CD3-, CD19-, i.e. on cells with the phenotype of mouse PDCs. Multi-color fluorescent staining of spleen cells clearly revealed, that all CD11c+ CD45R (B220)+ Ly-6C+ PDCs are mPDCA-1+ and that mPDCA-1 expression is restricted to PDCs in non-treated mice.
Downstream applications
The isolated PDCs are suitable for studies on their functional properties, e.g. antigen uptake and presentation, crosspriming of cytotoxic T cells, T cell activation or tolerance induction, or T helper cell polarization by PDCs in experimental mouse models of infection and autoimmunity.
Highly pure PDCs were isolated with Anti-mPDCA-1 MicroBeads and have been further analyzed for IDO-dependent tolerance-induction.1 Anti-mPDCA-1 MicroBeads have also been used to isolate PDCs for subsequent analysis of EBV-induced gene 3 (EBI3) expression and regulation in mouse DC subsets.2 |
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| Figure 1 |
| Mouse mPDCA-1+ PDCs were enriched from a mouse spleen cell suspension using Anti-mPDCA-1 MicroBeads, two MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with Anti-mPDCA-1-FITC and Anti-Ly-6C-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
| Spleen cells before separation |
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| Spleen cells depleted of mPDCA-1+ PDCs |
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| Enriched mPDCA-1+ PDCs |
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