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| Epidermal Langerhans Cell MicroBead Kit |
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| Overview |
| The Epidermal Langerhans Cell MicroBead Kit is designed for the positive selection or depletion of Langerhans cells (LCs) from single-cell suspensions of the epidermis. |
| Details |
Background information
Epidermal LCs are the principal dendritic cell population in the epidermis and play a pivotal role in the initiation and maintenance of primary immune responses in the skin. Immature LCs are highly specialized in antigen uptake. In vivo LCs exposed to pathogens migrate from the skin to the draining lymph nodes, where they elicit antigen-specific T cell responses.1-5
Applications
Positive selection or depletion of mouse epidermal Langerhans cells from single-cell suspension prepared from epidermis using the Epidermis Dissociation Kit, mouse. |
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| Figure 1 |
| A single-cell suspension was prepared using the Epidermis Dissociation Kit, mouse. Langerhans cells were isolated from mouse epidermis single-cell suspension by using the Epidermal Langerhans Cell MicroBead Kit, one MS Column, and a MiniMACS™ Separator. The cells were fluorescently stained with CD207 and CD11c and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
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| After separation |
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| Details |
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| Products |
| Epidermal Langerhans Cell MicroBead Kit, mouse |
- for 109 total cells
Download datasheet
130-095-408
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| References |
| 1. Takahara, K et al. (2002) Int. Immunol. 14: 433–444. |
| 2. Romani, N. et al. (2003) APMIS 111: 725–740. |
| 3. Schuler, G. and Steinman, R.M. (1985) J. Exp. Med. 161: 526–546. |
| 4. Merad, M. et al. (2008) Nature Reviews Immunology 8: 935–947. |
| 5. Kaplan, D.H. et al. (2008) Eur. J. Immunol. 38: 2369–2376. |
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