| Description |
| The CD4+ Dendritic Cell Isolation Kit is an indirect magnetic labeling system designed to isolate CD4+ DCs from single-cell suspensions of spleen. CD4+ DCs coexpress CD11c, CD11b, MHC class II, CD40, CD80, CD86 and are negative for CD8 and CD205. They represent the major population of DCs in spleen and comprise only a small population in peripheral lymph nodes (56% and 4% of all CD11c+ DCs, respectively). The isolation of CD4+ DCs is performed in two steps. First, DCs are enriched by depletion of T cells, NK cells, B cells, and granulocytes with a cocktail of biotin-conjugated antibodies against CD90 (Thy1.2), CD3, CD8a (Ly-2), CD49b (DX5), CD45R (B220), Gr-1 (Ly-6G/C) and Anti-Biotin MicroBeads. Subsequently, CD4+ DCs are positively selected from the enriched DC fraction with CD4 (L3T4) MicroBeads. |
| Applications |
| CD4+ DCs isolated using the CD4+ Dendritic Cell Isolation Kit are suitable to analyze antigen uptake, antigen processing, T cell activation or T cell tolerance induction, cross-priming of cytotoxic T cells, and T cell polarization by CD4+ DCs. |
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| Figure 1 |
| Isolation of CD4+ DCs from a mouse spleen cell suspension using the CD4+ Dendritic Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator. |
| Spleen cells before separation |
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| Enriched CD4+CD11c+ DCs |
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| Isolated CD4+CD11c+ DCs |
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