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| CD4+ Dendritic Cell Isolation Kit |
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| Overview |
| The CD4+ Dendritic Cell Isolation Kit is an indirect magnetic labeling system designed to isolate CD4+ DCs from single-cell suspensions of splenocytes. |
| Details |
Background information
CD4+ DCs coexpress CD11c, CD11b, MHC class II, CD40, CD80, CD86 and are negative for CD8 and CD205. They represent the major population of DCs in spleen and comprise only a small population in peripheral lymph nodes (56% and 4% of all CD11c+ DCs, respectively). The isolation of CD4+ DCs is performed in two steps.
Detailed separation procedure
First, DCs are enriched by depletion of T cells, NK cells, B cells, and granulocytes with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Subsequently, CD4+ DCs are positively selected from the enriched DC fraction with CD4 MicroBeads.
Downstream applications
CD4+ DCs isolated using the CD4+ Dendritic Cell Isolation Kit are suitable to analyze antigen uptake, antigen processing, T cell activation or T cell tolerance induction, cross-priming of cytotoxic T cells, and T cell polarization by CD4+ DCs. |
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| Figure 1 |
| Isolation of CD4+ DCs from a mouse spleen cell suspension using the CD4+ Dendritic Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator. |
| Spleen cells before separation |
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| Enriched CD4+CD11c+ DCs |
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| Isolated CD4+CD11c+ DCs |
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| Details |
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| Products |
| CD4+ Dendritic Cell Isolation Kit, mouse |
- for 2×109 total cells
Download datasheet
130-091-262
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