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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  for non-human primate cells
  for mouse cells
  Stem and progenitor cells
  Neural cells
  T cells
  NK cells
  B cells
  Monocytes and macrophages
  Myeloid derived suppressor cells
  Dendritic cells
  Antigen-presenting cells
  Granulocytes
  Leukocytes
  Endothelial cells
  Erythroid cells
  Cytokine-producing cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD4+ Dendritic Cell Isolation Kit
Overview
The CD4+ Dendritic Cell Isolation Kit is an indirect magnetic labeling system designed to isolate CD4+ DCs from single-cell suspensions of splenocytes.
Details
Background information
CD4+ DCs coexpress CD11c, CD11b, MHC class II, CD40, CD80, CD86 and are negative for CD8 and CD205. They represent the major population of DCs in spleen and comprise only a small population in peripheral lymph nodes (56% and 4% of all CD11c+ DCs, respectively). The isolation of CD4+ DCs is performed in two steps.

Detailed separation procedure
First, DCs are enriched by depletion of T cells, NK cells, B cells, and granulocytes with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Subsequently, CD4+ DCs are positively selected from the enriched DC fraction with CD4 MicroBeads.

Downstream applications
CD4+ DCs isolated using the CD4+ Dendritic Cell Isolation Kit are suitable to analyze antigen uptake, antigen processing, T cell activation or T cell tolerance induction, cross-priming of cytotoxic T cells, and T cell polarization by CD4+ DCs.
Columns
For the first magnetic separation (depletion): LD or autoMACS® Columns; for the second magnetic separation (positive selection): MS or autoMACS Columns.
 
Figure 1
Isolation of CD4+ DCs from a mouse spleen cell suspension using the CD4+ Dendritic Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator.
Spleen cells before separation
Enriched CD4+CD11c+ DCs
Isolated CD4+CD11c+ DCs
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Products
CD4+ Dendritic Cell Isolation Kit, mouse
- for 2×109 total cells
Download datasheet
130-091-262
Qty.:
 

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