CD11c MicroBeads
Description CD11c MicroBeads have been developed for the isolation of mouse DCs from single-cell suspensions of lymphoid and non-lymphoid tissues. Unlike in humans, in mice CD11c is expressed on all defined DC subsets. With CD11c MicroBeads, complicated procedures for the isolation of DCs can be replaced by a fast and simple positive selection strategy. Applications DCs isolated with CD11c MicroBeads have been used for phenotypical and functional analyses, e.g. T cell activation, polarization, and tolerance induction in different experimental mouse models. CD11c MicroBeads were used to isolate highly pure DCs from spleen and lymph nodes1,2, Peyer's patches3, colonic lamina propria4, bone marrow5, epidermis6, lung7, liver8, or brain9. Columns For positive selection: MS, LS, XS, or autoMACS® Columns; for depletion: LD, CS, D, or autoMACS Columns.		Figure 1 DCs were isolated from a mouse spleen cell suspension using CD11c MicroBeads, a MiniMACS™ Separator, and two MS Columns. Spleen cells before separation Isolated CD11c+ DCs Figure 2 CD11c+ DCs isolated with CD11c MicroBeads from mouse spleen were stained with CD11c-APC and counterstained with Anti-mPDCA-1-FITC (A), CD8-PE (B) and CD4-FITC (C) for analysis of subset distribution. A B C

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