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| Overview |
MACS® HSC-CFU Media have been developed to assess the differentiation potential of human hematopoietic stem and progenitor cells as Colony Forming Units (CFU). They are ideally suited for use with
- unseparated blood3, bone marrow4, or fetal liver cells
- sorted cell populations1,4
- or ES and iPS cell-derived hematopoietic precursors2
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| Details |
MACS® HSC-CFU Media are standardized semi-solid media based on methylcellulose in IMDM, supplemented with FBS, BSA, and different growth factors. MACS® HSC-CFU media are designed to maximize growth and differentiation of progenitor cells and allow the clonal progeny of a single cell to grow in a distinct cluster or colony. They are produced under tightly controlled manufacturing conditions and use highly qualified raw materials to provide a consistent and optimally performing colony assay.
MACS® HSC-CFU media are available in different formats. For details on media composition and supported colony types please see figure 2. |
| Certificate of Analysis |
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| Figure 1 |
| MACS® HSC-CFU Media. |
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| Figure 2 |
| Formulation of HSC-CFU media. |
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| Details |
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| References |
| 1. Del Fante et al. (2005) Stem Cells Dev. 14: 734–739. |
| 2. Chang et al. (2006) Blood 108: 1515–1523. |
| 3. Biedermann et al. (2007) Leuk. Res. 31: 211–220. |
| 4. Watts et al. (2008) Cytotherapy 10 (suppl. 1): poster no. 150. |
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