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| Overview |
| The CD200 antibody can be used for the identification and enumeration of CD200+ cells, e.g., cancer stem cells, by flow cytometry. |
| Details |
Background information
Clone OX-104 reacts with the human CD200 antigen, also known as MRC, MOX1/2, and OX-2, a type-1 membrane glycoprotein, which contains two immunoglobulin domains. Studies of the related gene in mouse and rat suggest that CD200 may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues1. CD200 may serve as a cancer stem cell marker as co-expression with other reported stem cell markers was found on prostate, breast, brain, and colon cancers2. In addition, CD200 expression was shown to be a prognostic factor in AML3 and multiple myeloma4. |
| Clone | Isotype |
| OX-104 | Mouse IgG1 |
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| Figure 1 |
| Human peripheral blood mononuclear cells (PBMCs) were stained with CD200 antibodies conjugated to PE (A) or APC (B) as well as with CD19-APC or CD19-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with CD200-Biotin were stained additionally with Anti-Biotin-PE and CD19-APC (C). Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
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| Favorites / Prices |
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| Details |
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| Products |
| CD200-PE, human |
- for 100 tests (1)
Download datasheet
130-096-819
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| CD200-APC, human |
- for 100 tests (1)
Download datasheet
130-096-816
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| CD200-Biotin, human |
- for 100 tests (2)
Download datasheet
130-096-817
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 µl. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 µl. |
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