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| Anti-Sca-1 MicroBead Kit (FITC) |
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| Overview |
| The Anti-Sca-1 MicroBead Kit (FITC) has been developed for the positive selection of hematopoietic stem and progenitor cells from mouse bone marrow. Sca-1+ stem cells have been isolated from peripheral blood1, fetal2, and adult liver3 as well as heart4 and prostate tissue5. The kit can be used either alone or in combination with the Lineage Cell Depletion Kit, mouse for enrichment of KSL cells as well as in combination with the CD105 MultiSort Kit (PE) for the enrichment of long-term repopulating hematopoietic stem cells (LT-HSCs). |
| Details |
Background information
Sca-1 (stem cell antigen-1) is one of the defining markers for KSL cells (c-kit+ Sca-1+ Lin-), the population that comprises the stem cell compartment in mouse bone marrow. Sca-1 expression in combination with CD105 can be further used to define long-term repopulating hematopoietic stem cells (LTR-HSCs)6,7.
The Sca-1 protein is encoded by one of the two Ly-6A/E alleles expressed in different mouse strains (Ly-6E.1: e.g. BALB/c, C3H, NZB; Ly-6A.2: e.g. C57BL/6, SJL, 129, AKR). The anti-Sca-1 monoclonal antibody included in the Sca-1 MicroBead Kit (FITC) recognizes both, Ly-6E.1 and Ly-6A.2.
Downstream applications
Bone marrow-derived Sca-1+ cells have been used for nonhematopoietic differentiation into hepatocytes in vivo8 and neural cells in vitro9. ES cell-derived SCA-1+ cells could be differentiated into endothelial cells with high purity and yield10. Furthermore, smooth muscle cells have been generated from ESCs via isolation of Sca-1+ cells11. |
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| Figure 1 |
| Isolation of Sca-1+ cells from mouse bone marrow using the Anti-Sca-1 MicroBead Kit (FITC), a MiniMACS™ Separator and two MS Columns. Cell debris and dead cells were excluded based on scatter signals and PI fluorescence. |
| A: Before separation |
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| B: Sca-1+ cells |
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| Details |
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| Products |
| Anti-Sca-1 MicroBead Kit (FITC), mouse |
- for 109 total cells
Download datasheet
130-092-529
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| References |
| 1. Yamada et al. (2004) J. Immunol. 172: 1266–1272. |
| 2. Cherqui et al. (2006) Stem cells 24: 44–54. |
| 3. Petersen et al. (2003) Hepatology 37: 632–640. |
| 4. Matsuura et al. (2004) JBC 279: 11384–11391. |
| 5. Burger et al. (2005) Proc. Natl. Acad. Sci. USA 102: 7180–7185. |
| 6. Chen et al. (2002) Proc. Natl. Acad. Sci. USA 99: 15468–15473. |
| 7. Chen et al. (2003) Immunity 19: 525–533. |
| 8. Lagasse et al. (2000) Nat. Med. 6: 1229–1234. |
| 9. Sanchez-Ramos et al. (2000) Exper. Neurol. 164: 247–256. |
| 10. Xiao et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26: 2244–2251. |
| 11. Zempetaki et al. (2007) Am. J. Physiol. Cell Physiol. 293: C1126–1238. |
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