CD90.2 MicroBeads ( #130-049-101 )

CD90.2 MicroBeads

Description

CD90.2 MicroBeads were developed for positive selection or depletion of mouse T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood.

Details

Background information
The mouse CD90.2 alloantigen, also known as Thy1.2, is a pan T cell marker for the most common inbred mouse strains. These strains include BALB/c, DBA, CBA/J, C3H, C57BL/6, NZB/-, S3L and others. CD90.2 is expressed on thymocytes, peripheral T cells, on some intraepithelial T cells, and at lower levels on early hematopoietic stem cells in bone marrow.
The mouse CD90.2 antibody does not cross-react with CD90.1 (Thy1.1).

Downstream applications
T cells isolated with CD90.2 MicroBeads were used to analyze cytokine expression in a mouse model of lung fibrosis¹ or after adoptive transfer of T cells from wild type and knock-out mice into immunodeficient mice.² Furthermore, CD90.2 MicroBeads were used for the isolation of tumor-infiltrating T cells to evaluate their immunotherapeutic potential³ or functional properties.⁴

Columns

For positive selection: MS, LS, XS, or autoMACS Columns. For depletion: LD, CS, D, or autoMACS Columns.

Figure 1

Isolation of CD90.2 (Thy1.2)⁺ T cells from a mouse spleen cell suspension using CD90.2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were stained with CD90.2-FITC.

A: Before separation

B: CD90.2^- cells

C: Isolated CD90.2⁺ cells