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Cell separation reagents Cell separation reagents
  for human cells
  for non-human primate cells
  for mouse cells
  Stem and progenitor cells
  Neural cells
  T cells
  NK cells
  B cells
  Monocytes and macrophages
  Myeloid derived suppressor cells
  Dendritic cells
  Antigen-presenting cells
  Granulocytes
  Leukocytes
  Endothelial cells
  Erythroid cells
  Cytokine-producing cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD11b MicroBeads
Description
Mouse CD11b MicroBeads are used for the depletion or positive selection of mouse (or human) macrophages and monocytes from lymphoid and non-lymphoid tissue or blood. CD11b is expressed on macrophages/monocytes and to a lower extent on granulocytes, NK cells, and subsets of dendritic cells.
Applications
CD11b MicroBeads have been used to isolate CD11b+ cells from cell suspensions of spleen1, lymph node2, peritoneal cavity3, liver4, muscle tissue2, or blood5. They were also used in combination with other MicroBeads for the enrichment of mast cells from peritoneal cell suspensions.3
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns; for depletion: LD, CS, D, or autoMACS Columns.
 
Figure 1
Positive selection of CD11b+ cells from a mouse spleen cell suspension using CD11b MicroBeads, an MS Column, and a MiniMACS™ Separator.
Spleen cells before separation
Spleen cells after depletion of CD11b+ cells
Isolated CD11b+ mouse cells
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Details
Products
CD11b MicroBeads, human and mouse
- for 1×109 total human cells, or for 2×109 total mouse cells
Download datasheet
130-049-601
Qty.:
 

Related products
CD11b antibodies
References
1. Lohoff et al. (1997) Immunity 6: 681–689.
2. Dupuis et al. (2000) J. Immunol. 165: 2850–2858.
3. Chen et al. (1999) Immunol. 96: 544–550.
4. Do et al. (1999) J. Biol. Chem. 274: 19587–19592.
5. Mathy et al. (2000) J. Immunol. 164: 4429–4432.
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