CD15 MicroBeads

Convenient: isolate or deplete CD15⁺ myeloid cells in a single step

Overview

CD15 MicroBeads were developed for positive selection or depletion of CD15⁺ cells from human lysed peripheral blood or single-cell suspensions from tissues.

Details

Background information
The CD15 antibody recognizes a 3-fucosyl-N-acetyllactosamine carbohydrate modification in glycolipids or glycoproteins. The CD15 antigen is expressed on neutrophils, eosinophils, and monoblastoid precursor cells of the myeloid lineage but not on basophils and lymphocytes. The antigen is also present on Hodgkin/Reed-Sternberg cells.

Downstream applications
CD15 MicroBeads are used for the isolation¹ or depletion of human eosinophils and neutrophils (basophils are CD15^-) from peripheral blood and from tissues such as ovarian carcinoma tissue.² Furthermore, CD15 MicroBeads can be used for the depletion of myeloid cells from bone marrow.

Columns

For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.

Figure 1

CD15⁺ cells were isolated from lysed human peripheral blood using CD15 MicroBeads,
an MS Column, and a MiniMACS™ Separator. Cells are fluorescently stained with CD15-FITC.

Before separation

CD15^– cells

Enriched CD15⁺ cells