CD38 MicroBead Kit ( #130-092-263 )

CD38 MicroBead Kit

Overview

The CD38 MicroBead Kit was developed for the positive isolation or depletion of CD38⁺ cells from human PBMCs, bone marrow or tissue. The CD38⁺ cells are indirectly magnetically labeled with CD38-Biotin and Anti-Biotin MicroBeads.

Details

Background information
CD38 is a 45 kDa multi-lineage type II transmembrane glycoprotein. It is expressed at varying levels on the majority of hematopoietic as well as on some nonhematopoietic cells. The expression level depends on the developmental state and the activation status. CD38 is absent on most primitive CD34⁺ stem cells and upregulated on CD34⁺ cells upon lymphocyte commitment.¹

Applications
The CD38 MicroBead Kit can be applied for positive selection or depletion of hematopoietic cell subsets expressing human CD38 antigen, such as CD38⁺B cells, T cells, and NK cells from PBMCs, bone marrow, or tissue. The kit is also suitable for the isolation of CD38⁺plasma cells from tissue, such as bone marrow.
The CD38 MicroBead Kit can also be used for depletion of CD38⁺cells to isolate CD38^–cell subsets, e.g., CD34⁺CD38^–stem cells in combination with the CD34 MicroBead Kit.

Columns

For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.

Figure 1

Positive selection of CD38⁺ cells from PBMCs using the CD38 MicroBead Kit, an MS Column, and a MiniMACS Separator. CD38⁺ cells labeled with the CD38 MicroBead Kit were fluorescently stained with Anti-Biotin-FITC and CD19-PE.

Before separation

Enriched CD38⁺ cells

Figure 2

Depletion of CD38⁺ cells from PBMCs using the CD38 MicroBead Kit, an LD Column, and a MidiMACS Separator. Cell fractions are fluorescently stained with Anti-Biotin-FITC and CD19-PE to detect cells labeled with the CD38 MicroBead Kit.

Before separation

PBMCs depleted of CD38⁺ cells

PBMCs depleted of CD38<sup>+</sup> cells