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| Overview |
| CD20 MicroBeads were developed for positive selection or depletion of human CD20+ B cells from different cell sources such as human PBMCs, bone marrow, cell cultures or lymphoid tissues. |
| Details |
Background information
CD20 is a non-glycosylated transmembrane protein of 33–37 kDa that becomes heavily phosphorylated upon activation1,2. It is expressed exclusively on B cells, including pre-B cells, naive, mature, and activated B cells, B cell blasts, and the majority of malignant B cells, but not on early B cell progenitors or plasma cells2,3.
Downstream applications
Purified CD20+ B cells may be used in cell biological studies, such as analysis of signal transduction, chemotaxis, allergen response, apoptosis, or in molecular studies, e.g., subset-specific microarrays. |
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| Figure 1 |
| B cells were separated from PBMCs using CD20 MicroBeads and the autoMACS™ Separator. |
| PBMCs before separation |
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| Enriched CD20+ cells |
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| Details |
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| References |
| 1. Chang et al. (1996) Applied Histochemistry 4: 1-15. |
| 2. Polyak et al. (2002) CD20 Workshop Panel report. In: Leucocyte typing VII, Oxford University Press. |
| 3. Countouriotis et al. (2002) Stem cells 20: 215-229. |
| 4. Cragg et al. (2002) Opposing properties of CD20 mAb. In: Leucocyte typing VII, Oxford University Press. |
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