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| Overview |
| CD44 MicroBeads have been specifically designed and optimized for the isolation or depletion of CD44+ cells from single-cell suspensions from solid tumors, including breast, pancreatic, colon, and prostate tissues or cancer cell lines. |
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Background information
CD44 is a marker for many types of cancer stem cells (CSC), including breast CSCs that possess higher tumorigenicity and metastatic potential1, colorectal2, pancreatic3, and prostate4,5 CSCs. In addition, expression was observed in several cancers as well as on carcinoma cell lines. Here, it plays a role in cancer cell migration and matrix adhesion in response to a cellular microenvironment, thus enhancing cellular aggregation and tumor cell growth6. CD44 is also expressed on mesodermal cells, such as hematopoietic, fibroblastic, and glial cells. |
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| Figure 1 |
| CD44+ cells were isolated from a mixture of U937 (CD44+) and 1881 (CD44–) cell lines. CD44+ cells were isolated using the CD44 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD44-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. |
| A: Before separation |
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B: CD44– cells
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| C: CD44+ cells |
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| Details |
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| References |
| 1. Al-Hajj et al. (2003) Proc. Natl. Acad. Sci. USA 100: 3983–3988. |
| 2. Dalerba et al. (2007) Proc. Natl. Acad. Sci. USA 104: 10158–10163. |
| 3. Li et al. (2007) Cancer Res. 67: 1030–1037. |
| 4. Patrawala et al. (2006) Oncogene 25: 1696–1708. |
| 5. Collins et al. (2005) Cancer Res. 65: 10946–10951. |
| 6. Aruffo et al. (1990) Cell 61: 1303–1313. |
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