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| Overview |
IL-3 stands for interleukin 3. Mouse IL-3 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Mouse IL-3 can be used for a variety of applications, including:
- As cell culture supplement to maintain hematopoietic cells or cell lines, such as the pro-B lymphoid cell line BaF/3 or 32Dcl3 cells.
- Induction of colony formation of hematopoietic progenitor cells in semi-solid medium.
- In vitro differentiation studies.
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| Details |
Background information
IL-3 is a hematopoietic growth factor which is produced mainly by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. The broad spectrum of biologic activities of IL-3 includes the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays. |
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