Human EGF ( #130-093-825 #130-093-826 #130-093-827 )

Human EGF

Overview

EGF stands for epidermal growth factor. Human EGF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Human EGF can be used as an ingredient for many cell culture media. EGF stimulates the proliferation and differentiation of a wide variety of cell types deriving both from ectoderm and mesoderm.

Details

Background information
EGF is the prototype of the large family of EGF-like proteins with a common structural motif comprising three intramolecular disulfide bonds. EGF is produced by various cell types like mammary gland cells, gut epithelial cells, and cells in the nervous system and the kidney. Production of EGF is induced by testosterone and inhibited by estrogens. EGF stimulates the proliferation and differentiation of mesenchymal cells, acts as a mitogen for fibroblasts, epithelial and endothelial cells, and promotes colony formation of epidermal cells.

Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Figure 1

In vitro generation of primary mouse neurospheres. A single-cell suspension was prepared from mouse brain using the Neural Tissue Dissociation Kit (P) and the gentleMACS™ Dissociator. Isolated Prominin-1⁺ cells were cultivated for 7 days in MACS NeuroMedium containing MACS Supplement B27 PLUS, Human FGF-2 (20 ng/mL), and Human EGF (20 ng/mL).

<b><i>In vitro</i> generation of primary mouse neurospheres.</b> A single-cell suspension was prepared from mouse brain using the Neural Tissue Dissociation Kit (P) and the gentleMACS™ Dissociator. Isolated Prominin-1<sup>+</sup> cells were cultivated for 7 days in MACS NeuroMedium containing MACS Supplement B27 PLUS, Human FGF-2 (20 ng/mL), and Human EGF (20 ng/mL).