MSC Research Tool Box - MSCA-1 (W8B2) ( #130-093-572 )

MSC Research Tool Box - MSCA-1 (W8B2)

Overview

The MSC Research Tool Box – MSCA-1 (W8B2), based on the antibody clone W8B2, was developed for the standardized and optimized enrichment and expansion of MSCs with a high proliferative capacity from bone marrow aspirates.

Details

Background information
The Anti-MSCA-1 (W8B2) MicroBeads recognize the mesenchymal stromal cell antigen-1 (MSCA-1), which is identical to tissue non-specific alkaline phosphatase¹.

MSCA-1 was shown to be restricted to MSCs in the CD271^brightpopulation in bone marrow². These CD271^brightCD45^dim MSCs have a much higher clonogenic capacity compared to the CD271⁺CD45⁺ fraction in bone marrow. Therefore, MSCA-1 is a highly specific marker for the isolation of MSCs with a high proliferative potential from bone marrow. MSCA-1 expression was not found on placenta-derived MSCs after culture³.

Antibodies against MSCA-1 show broad interspecies cross-reactivity such as dog, pig, sheep, goat and monkey⁴.

Detailed procedure
The tool box contains all necessary reagents for the magnetic isolation of MSCA-1⁺ cells, including Anti-MSCA-1 (W8B2) MicroBeads for magnetic labeling and FcR Blocking Reagent to block non-specific labeling via Fc receptors, NH Expansion Medium, a specialized medium for the expansion of MSCs, and CytoMix – MSC, a cytokine cocktail designed specifically for the optimal and reproducible expansion of MSCs.

Columns

MS, LS, XS, or autoMACS® Columns.

Further information

Stem Cell Research Brochure
[PDF; 3,8 MB]
Stem Cell Product List Sept 2011
[PDF; 709,8 KB]

Isolation of MSCs from bone marrow mononuclear cells (BM-MNCs) using Anti-MSCA-1 (W8B2) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained stained with Anti-MSCA-1 (W8B2)-APC and CD45-FITC.

Before separation

MSCA-1^– cells

MSCA-1⁺ cells