CD138+ Plasma Cell Isolation Kit ( #130-092-530 )

CD138⁺ Plasma Cell Isolation Kit

Description

The CD138⁺ Plasma Cell Isolation Kit was developed for the isolation of CD138⁺ CD45R(B220)^low/– CD19^low/– antibody-secreting plasma cells¹.
CD138 (Syndecan-1) is a heparan sulfate-rich integral membrane proteoglycan, which functions as a matrix receptor for interstitial collagens, fibronectin, and thrombospondin. CD138 is predominantly expressed on the surface of epithelial cells in mature mouse tissues.² Its expression on cells of the B cell lineage correlates with their developmental stage, location, and adhesion. In mice, CD138 is expressed on pre-B and immature B lymphocytes in the bone marrow. It is lost when B cells emigrate into the periphery, is absent on circulating and peripheral B cells, and is re-expressed upon B cell differentiation into plasmablasts and plasma cells.³ The kit is suitable to isolate highly pure CD138⁺ plasma cells from spleen, lymph nodes, and bone marrow.

Applications

CD138⁺ plasma cells isolated with the CD138⁺ Plasma Cell Isolation Kit can be used to analyze molecular pathways of signal transduction or gene expression profiling. Furthermore, they are suitable for studies on plasma cell dysfunctions, e.g. in allergy, asthma, autoimmunity, or infectious diseases and migrational behaviour of plasma cells.

Columns

For the first magnetic separation (depletion): LD or autoMACS™ Columns; for the second magnetic separation (positive selection): MS or autoMACS Columns.

Figure 1

Plasma cells were isolated from a mouse spleen cell suspension by using the CD138⁺Plasma Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ and a MiniMACS™ Separator. The cells were fluorescently stained with CD45R (B220)-APC, CD19-FITC, and CD138-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.

Spleen cells before separation

Pre-enriched plasma cells after depletion of CD49b (DX5)⁺ cells and CD45R (B220)⁺ cells

Isolated CD138⁺ plasma cells

Figure 2

Plasma cells were isolated from a mouse bone marrow cell suspension by using the CD138⁺ Plasma Cell Isolation Kit. The CD138⁺ plasma cells were stained intracellularly with goat anti-mouse IgG-PE by applying the solid-phase intracellular staining procedure (Inside Stain Kit # 130-090-477). Additionally, the cell surface of the CD138⁺ plasma cells was fluorescently stained with CD138-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.