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 - Reliable: excellent reproducibility avoids cumbersome and expensive experiment repetitions
- Proven: successfully applied in basic and translational research
- Straightforward: optimal for subsequent B cell subset isolation
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| Overview |
| The B Cell Isolation Kit was developed for the fast and efficient isolation untouched resting B cells from single-cell suspensions of lymphoid tissues. |
| Details |
Detailed separation procedure
Highly pure resting B cells are isolated by magnetic labeling and depletion of CD43-expressing B cells (activated B cells, plasma cells and CD5+ B-1a cells) and non-B cells. CD43+ B cells, T cells, NK cells, dendritic cells, macrophages, granulocytes, and erythroid cells are labeled with a cocktail of biotin-conjugated antibodies against CD43 (Ly-48), CD4 (L3T4), and Ter-119, as well as Anti-Biotin MicroBeads. The B Cell Isolation Kit includes the Biotin-Antibody Cocktail for labeling of non-target cells and Anti-Biotin MicroBeads.
Downstream applications
Untouched B cells may be used for further magnetic separation of B cells into subpopulations. B cells isolated with the B Cell Isolation Kit were e.g. analyzed for transgene expression by Northern blot analysis in a transgenic reporter model.1 |
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| Figure 1 |
| Isolation of untouched B cells from a mouse spleen single-cell suspension using the B Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column. |
| Before separation |
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| Isolated CD45R+ B cells |
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| Details |
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| References |
| 1. Harrow, F. and Ortiz, B.D. (2005) J. Immunol. 175: 6659–6667. |
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