| Description |
| The B Cell Isolation Kit is an indirect magnetic labeling system to isolate untouched resting B cells from single-cell suspensions of lymphoid tissues. Highly pure resting B cells are isolated by magnetic labeling and depletion of CD43-expressing B cells (activated B cells, plasma cells and CD5+ B-1a cells) and non-B cells. CD43+ B cells, T cells, NK cells, dendritic cells, macrophages, granulocytes, and erythroid cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies against CD43 (Ly-48), CD4 (L3T4), and Ter-119, as well as Anti-Biotin MicroBeads. The B Cell Isolation Kit includes the Biotin-Antibody Cocktail for labeling of non-target cells and Anti-Biotin MicroBeads. |
| Applications |
| Untouched naive B cells are used for functional studies where effects due to crosslinking of surface molecules should be excluded. Untouched B cells may also be used for further magnetic separation of B cells into subpopulations. B cells isolated with the B Cell Isolation Kit were e.g. analyzed for transgene expression by Northern blot analysis in a transgenic reporter model.1 |
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| Figure 1 |
| Isolation of untouched B cells from a mouse spleen cell suspension using the B Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column. |
| Spleen cells before separation |
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| Isolated CD45R+ B cells |
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