| Description |
| Mouse CD43 (Ly-48) MicroBeads are used to isolate untouched resting B cells (B-2 cells) from peripheral lymphoid tissue or of late B cell progenitors from bone marrow. The CD43 antigen is expressed on nearly all leukocytes except immature and mature resting B cells. By using CD43 MicroBeads, all CD43-expressing cells are magnetically labeled, whereas the resting B cell subset stays untouched and is collected in the column effluent. |
| Applications |
| Resting B cells isolated with CD43 MicroBeads have been used for various studies on B cell differentiation and related molecular processes. Resting B cells isolated from spleen were used for in vitro analyses on antibody class switching.1 They were separated for comparative analysis on B cell receptor signal transduction in wild type and immunodeficient mice2 or were used as starting material for studies on germinal center formation.3 |
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| Figure 1 |
| Isolation of untouched B cells from a mouse spleen cell suspension using CD43 MicroBeads, a MiniMACS™ Separator, and an MS Column. |
| Spleen cells before separation |
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| Isolated untouched B cells |
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