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| Overview |
| CD43 (Ly-48) MicroBeads were developed for the isolation of untouched resting B cells (B-2 cells) from peripheral lymphoid tissues or late B cell progenitors from bone marrow. |
| Details |
Background information
The CD43 antigen is expressed on nearly all leukocytes except immature and mature resting B cells.
Detailed separation procedure
By using CD43 MicroBeads, all CD43-expressing cells are magnetically labeled, whereas the resting B cell subset stays untouched and is collected in the column effluent.
Downstream applications
Resting B cells isolated with CD43 MicroBeads have been used for various studies on B cell differentiation and related molecular processes. Resting B cells isolated from spleen were used for in vitro analyses on antibody class switching.1 They were separated for comparative analysis on B cell receptor signal transduction in wild type and immunodeficient mice2 or were used as starting material for studies on germinal center formation.3 |
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| Figure 1 |
| Isolation of untouched B cells from a mouse spleen cell suspension using CD43 MicroBeads, a MiniMACS™ Separator, and an MS Column. |
| Spleen cells before separation |
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| Isolated untouched B cells |
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| Favorites / Prices |
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| Details |
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| Products |
| CD43 (Ly-48) MicroBeads, mouse |
- for 2×109 total cells
Download datasheet
130-049-801
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| CD43 (Ly-48) MicroBeads, mouse – lyophilized |
- for 2x109 total cells
Download datasheet
130-097-148
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| References |
| 1. Hein et al. (1998) J. Exp. Med. 188: 2369–2374. |
| 2. Xu et al. (2000) Int. Immunol. 12: 397–404. |
| 3. de Vinuesa et al. (2000) J. Exp. Med. 191: 485–493. |
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