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 - Reliable: excellent reproducibility avoids cumbersome and expensive experiment repetitions
- Proven: successfully applied in basic and translational research
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| Overview |
| CD19 MicroBeads were developed for the positive selection or depletion of mouse B cells from peripheral blood and various tissues such as bone marrow, lymph nodes, and spleen. |
| Details |
Background information
CD19 is expressed on B lineage cells throughout their development from the early pro-B cell stages onwards and is down-regulated upon terminal differentiation to plasma cells.
Downstream applications
B cells isolated with CD19 MicroBeads were used to study the accessibility of enhancer elements during B cell development by in vivo footprinting.1 Cell extracts prepared from purified B cells were used to analyze conditions for V(D)J recombination in a cell-free system.2 Furthermore, mRNA was isolated from selected B cells for microarray analyses to quantify transcription factor expression during B cell development.3 |
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| Figure 1 |
| Isolation of mouse B cells from a spleen cell suspension using CD19 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells are fluorescently stained with CD19-FITC and CD45R (B220)-PE. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
| Before separation |
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| Isolated CD19+ cells |
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| Details |
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| References |
| 1. Shaffer et al. (1997) Immunity 6: 131–143. |
| 2. Stanhope-Baker et al. (1996) Cell 85: 887–897. |
| 3. Portis and Longnecker (2003) J. Virol. 77: 105–114. |
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