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| Overview |
| Mouse CD5 (Ly-1) MicroBeads can be used for the positive selection or depletion of CD5+ B-1a cells or T cells from peripheral lymphoid organs, non-lymphoid tissue or blood. |
| Details |
Background information
The CD5 antigen is expressed on B-1a cells but not on conventional B-2 cells. B-1 cells differ from B-2 cells with regard to origin, development, and tissue distribution and show an antigen-restricted B cell receptor repertoire. CD5 is also highly expressed on T cells.
Applications
For the isolation of highly pure B-1a cells by positive selection using CD5 MicroBeads, T cells have to be depleted in advance by using CD90.2 MicroBeads.
CD5 MicroBeads can also be used for the positive selection of T cells and subsequent analysis of cytokine secretion kinetics during T cell differentiation. |
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| Figure 1 |
| Positive selection of CD5+ cells from a mouse spleen cell suspension using CD5 (Ly-1) MicroBeads, a MiniMACS™ Separator, and an MS Column. |
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| Details |
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| Products |
| CD5 (Ly-1) MicroBeads, mouse |
- for 2×109 total cells
Download datasheet
130-049-301
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| References |
| 1. Hoerauf et al. (1994) Int. Immunol. 6: 1117–1124. |
| 2. Leitges et al. (1996) Science 273: 788–791. |
| 3. Taki et al. (1997) Immunity 6: 673–679. |
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