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| Overview |
| The antibody is suitable for the identification and enumeration of human CD73+ cells by flow cytometry and fluorescence microscopy. |
| Details |
Background information
The CD73 antigen is a 70 kDa membrane-bound enzyme that catalyzes the conversion of AMP to bioactive adenosine at neutral pH. This glycoprotein is also called ecto-5’-nucleotidase. CD73 is present on subsets of T and B lymphozytes, follicular dendritic cells, endothelial cells and marrow stromal cells (MSCs).1,2 |
| Clone | Isotype |
| AD2 | mouse IgG1 |
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| Human PBMCs were stained with CD73 antibodies conjugated to -PE (A) or APC (B) as well as with CD45-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells stained with CD73-Biotin (C) were also stained with Anti-Biotin-PE. |
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| B |
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| C |
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| Favorites / Prices |
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| Details |
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| Products |
| CD73-PE, human |
- for 100 tests (1)
Download datasheet
130-095-182
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| CD73-APC, human |
- for 100 tests (1)
Download datasheet
130-095-183
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| CD73-Biotin, human |
- for 100 tests (2)
Download datasheet
130-095-184
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| CD73 pure, human |
- 100 µg in 1 mL
Download datasheet
130-095-185
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 μL. |
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| References |
| 1. Thomson, L.F. et al. (1990). Tissue Antigens 35: 9-19. |
| 2. Pittenger, M.F. et al (1999) Science 284: 143-147. |
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