CD105 ( #130-094-926 #130-094-916 #130-094-941 )

CD105

Overview

The CD105 antibodies have been designed for the identification and enumeration of human CD105⁺ cells by flow cytometry or fluorescence microscopy. They are ideally suited for the evaluation of MACS Separations, e.g., human endothelial cells isolated with CD105 MicroBeads.

Details

Background information
The CD105 antigen, also known as endoglin, serves as a receptor for the growth and differentiation factors TGF-β1 and TGF-β3. An epitope of CD105 is recognized by the SH-2 antibody¹, which was raised against human mesenchymal stromal cells (MSC) that show mesodermal differentiation capacity.² Therefore, it can be used for studies on mesengenesis. CD105 is also expressed on mature endothelial cells and on some leukemic cells of B lymphoid and myeloid origin.

Clone	Isotype
43A4E1	mouse IgG1

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were spiked with human umbilical vein endothelial cells (HUVECs) and stained with CD105 antibodies conjugated to PE (A) or APC (B) as well as with CD45-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with CD105-Biotin (C) were stained with Anti-Biotin-PE as well as CD45-FITC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.