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| Overview |
The CD105 MultiSort Kit (PE), mouse has been developed for the isolation of CD105+ cells or cell subsets, including - vascular endothelial cells from mouse tissue or cell cultures
- long-term repopulating hematopoietic stem cells (LTR-HSCs) from mouse bone marrow when used in combination with the Anti-Sca-1 MicroBead Kit (FITC).
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| Details |
Background information
CD105, also known as Endoglin, is abundantly expressed on angiogenic endothelial cells. In mouse bone marrow, CD105 is also expressed on a subpopulation of Sca-1+ hematopoietic stem cells that displays long-term repopulating activity in lethally irradiated mice (LTR-HSCs)1.
Detailed separation procedure
In the first step, CD105+ cells are positively selected after labeling with the CD105 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD105 antibody. The selected CD105+ cells can be magnetically labeled and sorted according to a second antigen. The kits contains CD105 MultiSort MicroBeads, MultiSort Release Reagent, and the MultiSort Stop Reagent.
Downstream applications
Isolated CD105+Sca-1+ cells can be transplanted into lethally irradiated mice to evaluate the long-term engraftment potential of this cell population. |
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| Figure 1 |
| Isolation of CD105+Sca-1+ LTR-HSCs from mouse bone marrow using the CD105 MultiSort Kit (PE), mouse, and two LS Columns for the first positive selection followed by the Anti-Sca-1 MicroBead Kit (FITC), mouse, and two MS Columns in the second positive selection step. |
| A: Before separation |
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B: CD105+ cells
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| C: CD105+Sca-1+ LTR-HSCs |
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| Favorites / Prices |
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| Details |
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| Products |
| CD105 MultiSort Kit (PE), mouse |
- for 109 total cells
Download datasheet
130-092-924
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| References |
| 1. Chen et al. (2002) Proc. Natl. Acad. Sci. USA 99: 15468–15473. |
| 2. Chen et al. (2003) Immunity 19: 525–533. |
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