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| Overview |
| The CD146 MicroBead Kit was developed for the isolation of CD146+ cells. The kit consists of CD146 MicroBeads and FcR Blocking Reagent. The CD146 MicroBeads recognize the human CD146 antigen, also known as MUC18, MCAM, Mel-CAM, and S-Endo-1. |
| Details |
Background information
CD146 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily and contains five extracellular immunoglobulin (Ig)-like domains.1,2
CD146 possesses a limited tissue expression distribution, including endothelial cells, smooth muscle cells, pericytes, follicular dendritic cells, melanoma cells, and a subpopulation of activated T lymphocytes.1,3 CD146+ cells isolated from umbilical cord4, lipoaspirate5, dental pulp, or endometrial tissue6, are described to contain cells with clonogenic fibroblastic colony (CFU-F) activity and multilineage differentiation potential, which are often called mesenchymal or marrow stromal cells (MSCs).
CD146 functions as a Ca2+-independent cell adhesion molecule, which is involved in heterophilic cell-cell interactions7, and may be involved in the extravasion and/or homing of activated T cells3. CD146 expression in melanoma is also directly associated with tumor growth and metastasis.7,8
Applications
The CD146 MicroBead Kit can be used for:
- isolation of human mesenchymal stromal cells (MSCs) from lipoaspirate and other tissue sources,
- isolation of endothelial cells and pericytes from human endothelial tissue,
- isolation of human umbilical vein endothelial cells (HUVECs) and human umbilical cord perivascular cells (HUCPVCs)4 from umbilical cord vein.
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| Columns |
| For positive selection: LS, or XS Columns. |
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| Figure 1 |
| CD146+ cells were separated from lipoaspirate using the CD146 MicroBead Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD146-APC and CD34-FITC. |
| Before separation |
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| CD146– cell fraction |
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| CD146+ cell fraction |
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| Details |
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| References |
| 1. Bardin et al. (2001) Blood 98: 3677–3684. |
| 2. Yan et al. (2003) Blood 102: 184–191. |
| 3. Pickl et al. (1997) J. Immunol. 158: 2107–2115. |
| 4. Baksh et al. (2007) Stem Cells 25:1384–1392. |
| 5. Zannettino et al. (2008) J. Cell Physiol. 214: 413–421. |
| 6. Schwab and Gargett (2005) Reprod. Fert. Devel. 17: 111–111. |
| 7. Xie, S. et al. (1997) Cancer Res. 57: 2295–2303. |
| 8. Satyamoorthy et al. (2001) Oncogene 20: 4676–4684. |
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