|
|
 |
| Anti-MSCA-1 (W8B2) MicroBead Kit |
|
| Overview |
| The Anti-MSCA-1 (W8B2) MicroBead Kit, based on the antibody clone W8B2, was developed for the positive selection of mesenchymal stromal cells (MSCs) with a high proliferative capacity, derived from bone marrow aspirates. |
| Details |
Background information
The Anti-MSCA-1 (W8B2) MicroBeads recognize the mesenchymal stromal cell antigen-1 (MSCA-1), which is identical to tissue non-specific alkaline phosphatase1.
MSCA-1 was shown to be restricted to MSCs in the CD271bright population in human bone marrow2. These CD271brightCD45dim MSCs have a much higher clonogenic capacity compared to the CD271+CD45+ fraction in bone marrow. Therefore, MSCA-1 is a highly specific marker to isolate MSCs with a high proliferative potential from bone marrow. MSCA-1 expression was not found on placenta-derived MSCs after culture3.
Antibodies against MSCA-1 show broad interspecies cross-reactivity, such as dog, pig, sheep, goat and monkey4. |
| |
|
| MSCA-1 (W8B2)+ cells were isolated from human bone marrow mononuclear cells (BM-MNCs) using the Anti-MSCA-1 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were stained with Anti-MSCA-1 (W8B2)-APC and CD45-FITC. |
| Before separation |
 |
| MSCA-1– cells |
 |
| MSCA-1+ cells |
 |
|
|
|
|
| Favorites / Prices |
Request prices or add products to your favorite list.
|
| Details |
|
| Products |
| Anti-MSCA-1 (W8B2) MicroBead Kit, human |
- for 109 total cells
Download datasheet
130-093-583
|
|
| References |
| 1. Sobiesiak, M. et al. (2010) Stem Cells and Dev. 19 (5): 669-677 |
| 2. Bühring et al. (2007) Ann. NY Acad. Sci. 1106: 262–271. |
| 3. Battula, V. L. et al. (2007) Differentiation 75: 279–291. |
| 4. Rozemuller, H. et al. (2010) Stem Cells and Dev. 19 (12): 1911-1921. |
|
| MACS Stem Cell newsletter |
|
Stay current with important developments in stem cell research by subscribing to
Stem Cell Updates.
|
|
© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
