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| Overview |
| The Dead Cell Removal Kit is a fast and straightforward way of eliminating dead cells from cell cultures or tissue preparations. The Dead Cell Removal Kit contains ready-to-use MicroBeads and Binding Buffer for the magnetic labeling of cell debris, dead cells, and dying cells. The magnetically labeled material is removed by magnetic separation and pure, viable cells are obtained within 25 minutes. |
| Details |
Applications
The Dead Cell Removal Kit has been successfully used for the following applications:
- Removal of dead cells prior to MACS® Separation of cells
- Reduction of flow sorting time by elimination of dead cells
- Elimination of dead cells from cell cultures1
- Improvement of immunocytochemical analysis of frozen cells2
- Preparation of viable, single-cell suspensions from tissue
- Removal of dead cells from spermatozoa samples3
- Removal of dead cells prior to or following transfection of cells
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| Figure 1 |
| Working scheme for the Dead Cell Removal Kit. |
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| Figure 2 |
| Dead cells were eliminated from cultured cells (Jurkat cells) by labeling of cells with Dead Cell Removal MicroBeads and separation over an LS Column in the magnetic field of a MidiMACS™ Separator. Dead cells were fluorescently stained with propidium iodide. |
| A: Before separation |
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| B: Dead cell-depleted fraction |
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| Details |
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| References |
| 1. Kinoshita et al. (2002) J. Immunol. 169: 6187–6192. |
| 2. Gachard et al. (2000), Application Note. |
| 3. Glander et al. (2002) Andrologia 34: 360–366. |
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