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| Overview |
SDF-1 stands for stromal cell-derived factor-1, also termed CXCL12 or CXC motif chemokine 12. Human SDF-1α is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Human SDF-1α can be used for a variety of applications, including:
- Migration and homing assays.
- Studies on tumorigenesis, neuronal development, organogenesis, and autoimmune diseases.
- In vitro angiogenesis assays (cell outgrowth, proliferation, and tube formation).
- Adhesion assays.
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| Details |
Background information
SDF-1 is a small cytokine with chemotactic activity. The sequence of SDF-1 is highly conserved among the species. Mouse SDF-1 differs only in one amino acid from human SDF-1 and therefore shows cross-reactivity. SDF-1 binds to the G-protein-coupled seven-transmembrane receptor CXCR4 and induces a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. CXCR7 has been identified as another seven-transmembrane receptor for SDF-1, suggesting a role for SDF-1 in several aspects of tumorigenesis. In vitro, SDF-1 is a potent chemoattractant for T lymphocytes and monocytes, but not for neutrophils. Furthermore SDF-1 acts as a chemoattractant for CD34+ human hematopoietic progenitor cells in vivo and in vitro, as well as for mesenchymal stem cells in vitro. SDF-1 is implicated in neuronal migration, neural development, brain inflammation, and tumor growth in vivo.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays. |
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| Figure 1 |
| Human SDF-1α activity assay. The biological activity of Human SDF-1α was determined by transwell chemotaxis assay using U-937 cells. Migration rates are expressed as a percentage of the starting cell population. Activity of Human SDF-1α, research grade (red line) was compared to another commercially availabele product (black line). Similar effects were observed when using Jurkat cells. |
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| Figure 2 |
| SDS-PAGE of Human SDF-1α, research grade under reduced (R) and non-reduced (NR) conditions. |
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