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| Overview |
M-CSF stands for macrophage-colony stimulating factor. Human M-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Human M-CSF can be used for a variety of applications, including:
- Survival studies and apoptosis assays, for example, using peripheral blood monocytes.
- Differentiation of macrophages from peripheral blood monocytes.
- Differentiation of osteoclasts from CD14+ monocytes.
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| Details |
Background information
M-CSF, a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de). |
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| Figure 1 |
| Human M-CSF activity assay. The biological activity of Human M-CSF is determined by proliferation assay using NFS-60 cells. |
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| Figure 2 |
| SDS-PAGE of Human M-CSF, premium grade under reduced (R) and non-reduced (NR) conditions. |
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