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 - Effective: excellent NK cell purity
- Trouble-free: isolated NK cells are fully functional
- Unrivaled: superior T cell depletion
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| Description |
The NK Cell Isolation Kit II was developed for the isolation of untouched NK cells from single-cell suspensions of murine spleen, liver, or lymph nodes. Non-NK cells, i.e. T cells, dendritic cells, B cells, granulocytes, macrophages, and erythroid cells are magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure unlabeled NK cells is achieved by depletion of non-target cells.
This product replaces the NK Cell Isolation Kit, mouse #130-090-864. |
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Downstream applications
NK cells isolated using the NK Cell Isolation Kit II can be used for in vitro and in vivo applications, e.g. functional studies or adoptive transfer experiments. |
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| Figure 1 |
| Isolation of untouched NK cells from a BALB/c mouse spleen cell suspension using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD49b (DX5)-PE and CD3ε-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis. |
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| Isolated NK cells |
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| Figure 2 |
| Isolation of untouched NK cells from a C57BL/6 mouse spleen cell suspension using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD49b (DX5)-APC and CD3ε-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis. |
| Before separation |
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| NK cells after separation |
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| Details |
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| Products |
| NK Cell Isolation Kit II, mouse |
- for up to 109 total cells
Download datasheet
130-096-892
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© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
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