| Description |
The NK Cell Isolation Kit is an indirect magnetic labeling system for the isolation of untouched NK cells from single-cell suspensions of mouse lymphoid tissues. Highly pure untouched NK cells are isolated by depletion of non-NK cells. Non-NK cells, i.e. B cells, T cells, dendritic cells, macrophages, granulocytes, and erythroid cells, are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies against CD19, CD4 (L3T4), CD8a (Ly-2), CD5 (Ly-1), Gr-1 (Ly-6G/C), and Ter-119, as well as Anti-Biotin MicroBeads. Isolation of NK cells is achieved by depletion of the magnetically labeled cells.
The NK Cell Isolation Kit includes the Biotin-Antibody Cocktail for labeling of non-NK cells and Anti-Biotin MicroBeads. |
| Applications |
| Untouched NK cells are used for functional studies where effects due to crosslinking of surface molecules should be excluded. They may also be used for further magnetic separation of NK cell subsets according to expression of subset-specific markers. NK cells isolated with the NK Cell Isolation Kit were used to evaluate NK cell cytotoxicity in an experimental mouse model of AML.1 NK cells were also isolated from tumor tissues and subsequently analyzed for their cytotoxicity. In the same study NK cells were isolated from splenocytes and lymphnodes, and the effect of LIGHT on NK cell proliferation and IFN-Îł production by NK cells as well as the effect of NK cells on CD8+ T cell activation was analyzed.2 |
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| Figure 1 |
| Isolation of untouched NK cells from a mouse spleen cell suspension using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. |
| Spleen cells before separation |
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| Isolated CD49b (DX5)+ NK cells |
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