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 - Efficient: outstanding yields of purified NK cells
- Gentle: excellent cell viability
- Broadening your research horizon: isolated cells are suited for adoptive transfer
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| Description |
| Mouse CD49b (DX5) MicroBeads are used for the isolation of mouse NK cells from single-cell suspensions of lymphoid and non-lymphoid tissue, as well as from peripheral blood or body fluids. |
| Details |
Background information
CD49b is expressed on NK cells and a small population of T cells (CD4+CD3+ TCRa/ß+). These cells can be depleted prior to NK cell enrichment by using CD90.2 MicroBeads or CD5 (Ly-1) MicroBeads.
CD49b is less mouse strain–specific than other NK cell markers (e.g. NK1.1) and is expressed by the most common inbred mouse strains.
Downstream applications
NK cells isolated with CD49b (DX5) MicroBeads from wild-type or immunodeficient mice were used to study NK cell cytotoxicity in vitro1 or after adoptive transfer of isolated NK cells in vivo2. Furthermore, isolated NK cells were used to evaluate their role in experimental mouse models of immune-mediated liver injury3 and chronic infection4.
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| Figure 1 |
| Isolation of mouse NK cells from a mouse spleen cell suspension using CD49b (DX5) MicroBeads, a MiniMACS™ Separator, and an MS Column. |
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| Details |
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| Products |
| CD49b (DX5) MicroBeads, mouse |
- for 2×109 total cells
Download datasheet
130-052-501
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| References |
| 1. Tomasello et al. (2000) Immunity 13: 335–364. |
| 2. Shi et al. (2000) J. Immunol. 165: 3099–3104. |
| 3. Mühlen et al. (2004) J. Immunol. 172: 3034–3041. |
| 4. Hsieh et al. (2004) J. Immunol. 173: 2699–2704. |
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