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 - Fast: isolate untouched stem cells in less than one hour
- Straightforward: fully compatible with subsequent flow analysis and different sorting procedures
- Reliable: the ready-to-use cocktail minimizes experimental variation
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| Overview |
| The Lineage Cell Depletion Kit, mouse has been developed for efficient depletion of mature hematopoietic cells such as T cells, B cells, monocytes/macrophages, granulocytes, erythrocytes and their committed precursors. The kit allows the untouched isolation of lineage-negative cells from mouse bone marrow samples in less than an hour. |
| Details |
Detailed separation procedure
For depletion, cells are magnetically labeled with a cocktail of biotinylated antibodies against a panel of so-called lineage antigens (CD5, CD45R (B220), CD11b, Gr-1 (Ly-6G/C), 7-4, and Ter-119) and Anti-Biotin MicroBeads.
Downstream applications
The labeling procedure leaves lineage-negative cells untouched, allowing further magnetic separation of lineage-negative cells according to expression of markers such as CD1171,2 or Sca-13. The Lineage Cell Depletion Kit has been used for the enrichment of both, hematopoietic stem cells (HSCs)4 and marrow stromal cells (MSCs) from bone marrow5. |
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| Details |
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| Products |
| Lineage Cell Depletion Kit, mouse |
- for 109 total cells
Download datasheet
130-090-858
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| References |
| 1. Orlic (2002) Int. J. Hematol. 76 (Suppl. 1): 144–145. |
| 2. Orlic et al. (2001) Proc. Natl. Acad. Sci. USA 98: 10344–10349. |
| 3. Lagasse et al. (2000) Nat. Med. 6: 1229–1234. |
| 4. Tang et al. (2006) Blood 108: 1189–1197. |
| 5. Forte et al. (2006) Stem cells 24: 23–33. |
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