CD34 MultiSort Kit ( #130-056-701 )

CD34 MultiSort Kit

Overview

The CD34 MultiSort Kit has been developed for the positive selection of CD34⁺ subpopulations by sequential sorting. CD34⁺hematopoietic stem and progenitor cells can be isolated from

peripheral blood,
bone marrow aspirate,
leukapheresis product,
cord blood,
differentiated ES and iPS cell cultures¹.

Details

Detailed separation procedure
Using the CD34 MultiSort Kit, in the first step CD34⁺ cells are positively selected after labeling with the CD34 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD34 antibody. The isolated CD34⁺ cells can be magnetically labeled and sorted according to a second antigen. The kit contains CD34 MultiSort MicroBeads, MultiSort Release Reagent, and MultiSort Stop Reagent.
Control staining after separation requires a monoclonal CD34 antibody other than QBEND/10, e.g. clone AC136

Downstream applications
The CD34 MultiSort Kit is used to isolate specific subsets of CD34⁺ hematopoietic stem and progenitor cells. CD34-selected cells can be further sorted according to their expression of, e.g., HLA-DR, CD43, CD45RA, CD61, CD71, CD90 (Thy1), or CD133.

Columns

MS, LS, XS, or autoMACS Columns.

Clone	Isotype
QBEND/10	Mouse IgG1

Further information

Isolation of CD34⁺HLA-DR^- cells
[PDF; 71,6 KB]
Isolation of CD34⁺CD38^- cells
[PDF; 75,4 KB]

Figure 1

CD34⁺HLA-DR^– cells isolated from human PBMCs using the CD34 MultiSort Kit. Positive selection of CD34⁺ cells from PBMCs was followed by the depletion of CD34⁺HLA-DR⁺ cells using HLA-DR-FITC and Anti-FITC MicroBeads resulting in a population of CD34⁺HLA-DR^– cells (right panel, red).

A: PBMCs before separation

B: CD34⁺ cells

C: CD34⁺HLA-DR^- cells

CD34<sup>+</sup>HLA-DR<sup>-</sup> cells

Figure 2

Step-by-step CD34 MultiSort separation procedure.

Figure 3

CD34⁺ lin^- cells isolated from peripheral blood using CD34 MultiSort Kit, May-Grünwald-Giemsa stained. (Courtesy of Dr. Ziegler, Tübingen, Germany.)

CD34<sup>+</sup> lin<sup>-</sup> cells isolated from peripheral blood using CD34 MultiSort Kit, May-Grünwald-Giemsa stained. (Courtesy of Dr. Ziegler, Tübingen, Germany.)