Lineage Cell Depletion Kit ( #130-092-211 )

Lineage Cell Depletion Kit

Description

The Lineage Cell Depletion Kit is an indirect magnetic labeling system for the depletion of mature hematopoietic cells such as T cells, B cells, NK cells, dendritic cells, monocytes, granulocytes, erythroid cells, and their committed precursors from bone marrow aspirate, cord blood, or mobilized leukapheresis product. The depletion of lineage-committed cells results in the enrichment of untouched stem and progenitor cells.

Details

Detailed separation procedure
Cells are first labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens (CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a (Glycophorin A)). These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells.

Downstream applications

Flow cytometric characterization of rare stem and progenitor cell subsets
Evaluation of hematopoietic differentiation potential in vitro using CFU assays (CFC, CFU-GM, BFU-E, and CFU-Mk)
Evaluation of the CFU-F potential

Columns

MS, LS, XS, or autoMACS® Columns.

Figure 1

Isolation of untouched lineage-negative cells from bone marrow mononuclear cells (BM-MNCs) using the Lineage Cell Depletion Kit and an LS Column. Cells were fluorescently stained with CD34-FITC.

BM-MNCs before separation

Isolation of untouched lineage-negative cells from bone marrow mononuclear cells (BM-MNCs) using the Lineage Cell Depletion Kit and an <a href="/en/PG_115_163_LS_Columns.aspx">LS Column</a>. Cells were fluorescently stained with <a href="/en/PG_595_204_CD34.aspx">CD34-FITC</a>.

Lineage⁺ fraction

CD34⁺ cells in the lineage cell–depleted fraction.