Indirect CD34 MicroBead Kit ( #130-046-701 )

Indirect CD34 MicroBead Kit

Overview

The Indirect CD34 MicroBead Kit was developed for the isolation of CD34⁺ precursor cells, using a two-step procedure involving indirect magnetic labeling from

peripheral blood
bone marrow
leukapheresis product
cord blood
differentiated-ES and iPS cells

Even faster, one-step isolation of CD34⁺cells can be achieved with the CD34 MicroBead Kit.

Details

Background information
CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts^1,2, endothelial progenitor cells^1,3-5, and mature endothelial cells⁴.

Detailed separation procedure
First, cells are labeled with the hapten-modified CD34 primary antibody QBEND/10⁶. In a second step, antibody-labeled cells are magnetically labeled with Anti-Hapten MicroBeads.

Control staining of cells isolated with the Indirect CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g. clone AC136

Downstream applications

Transplantation of CD34⁺ cells into humanized mouse models
Expansion and differentiation in culture
Reprogramming of CD34⁺ HSCs into iPS cells
Differentiation of ES or iPS cell-derived CD34⁺ -derived precursors into endothelial or hematopoietic lineages
Evaluation of differentiation potential in CFU assays
Characterization of surface marker expression by flow cytometry.

Columns

MS, LS, XS, or autoMACS® Columns.

Clone	Isotype
QBEND/10	Mouse IgG1

Figure 1

May-Grünwald-Giemsa–stained CD34⁺ cells isolated from bone marrow aspirate using the Indirect CD34 MicroBead Kit, an MS Column and a MiniMACS™ Separator.

May-Grünwald-Giemsa–stained CD34<sup>+</sup> cells isolated from bone marrow aspirate using the Indirect CD34 MicroBead Kit, an <a href="/en/PG_115_162_MS_Columns.aspx">MS Column</a> and a MiniMACS™ Separator.