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| Diamond CD34 Isolation Kit |
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| Overview |
| The Diamond CD34 Isolation Kit has been specifically developed for the isolation of highly pure CD34+Lin– hematopoietic stem cells from peripheral blood, bone marrow or cord blood. |
| Details |
Background information
The CD34 antigen is the most common marker for the isolation of hematopoietic stem and progenitor cells, but is also expressed on some lineage-committed cells. With the Diamond CD34 Isolation Kit, lineage-positive cells are removed in order to obtain pure, primitive CD34+ HSCs.
Detailed separation procedure
The Diamond CD34 MicroBead Kit includes a lineage depletion followed by a CD34 isolation. In the first step, cells are labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens. These cells are subsequently labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells. Then CD34+ cells are isolated from the lineage-negative stem and progenitor cells using CD34 MicroBeads. The resulting population obtained are CD34+Lin– stem cells.
Downstream applications
Purified CD34+Lin- stem cells can be used for many applications, including:
- gene or miRNA expression profiling
- characterization of undifferentiated stem cells and their developmental potential
- stimulation of proliferation and maturation by cytokines
- HSC-CFU assays
- adoptive transfer and hematopoietic reconstitution
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