| Description |
| The CD8a+ T Cell Isolation Kit is an indirect magnetic labeling system for the isolation of untouched cytotoxic CD8a+ T cells from single-cell suspensions of spleen and lymph nodes. Highly pure untouched CD8a+ T cells are isolated by depletion of non-CD8a+ T cells. Non-CD8a+ T cells, i.e. T helper cells, B cells, NK cells, dendritic cells, macrophages, granulocytes, and erythroid cells, are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies against CD4 (L3T4), CD45R (B220), CD49b (DX5), CD11b (Mac-1), and Ter-119, as well as Anti-Biotin MicroBeads. Isolation of CD8a+ cytotoxic T cells is achieved by depletion of the magnetically labeled cells. |
| Applications |
| Untouched CD8a+ cytotoxic T cells are used for functional studies where effects due to crosslinking of surface molecules should be excluded or if further magnetic separation of CD8a+ T cells into subpopulations is intended, e.g. for the isolation of naive CD8a+ T cells.1 CD8a+ T cells isolated with the CD8a+ T Cell Isolation Kit were used for adoptive transfer to evaluate the migration and proliferation properties of transferred T cells and to evaluate how to increase the function of low-avidity tumor-specific T cells in mouse tumor models2,3. Isolated CD8a+ T cells were also used to analyze the role of OX40 for the survival of primed CD8 T cells after adoptive transfer into tumor-challenged mice.4 In addition, CD8a+ T cells were isolated from tumor tissues and further analyzed for their cytotoxicity.5 |
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| Figure 1 |
| Isolation of untouched CD8a+ cytotoxic T cells from a mouse spleen cell suspension using the CD8a+ T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. |
| Spleen cells before separation |
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| Isolated CD8a+ T cells |
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