| Description |
The CD4+CD25+ Regulatory T Cell Isolation Kit has been developed for the isolation of CD4+CD25+ regulatory mouse T cells from single-cell suspensions of spleen and lymphnodes. CD4+CD25+ immunoregulatory T cells have been shown to actively suppress immune responses against autologous and foreign antigens in vivo and in vitro. CD25, the IL-2Rα chain, is also expressed on activated CD4+ T cells, CD8+ T cells, dendritic cells, and B cells.
The kit contains a cocktail of lineage specific biotin-conjugated antibodies against CD8 (Ly-2), CD11b (Mac-1), CD45R (B220), CD49b (DX5), Ter-119, and Anti-Biotin MicroBeads for depletion of non-CD4+ T cells, as well as CD25-PE and Anti-PE MicroBeads for subsequent positive selection of CD4+CD25+ regulatory T cells. |
| Applications |
| Regulatory T cells isolated from mouse lymph nodes with the CD4+CD25+ Regulatory T Cell Isolation Kit were used in coculture experiments with dendritic cells to study priming of dendritic cells for tolerance induction in vitro and after adoptive transfer of primed dendritic cells in vivo.1 Furthermore, they were isolated and used in various models for adoptive transfer experiments2, e.g. in an experimental autoimmune thyroiditis mouse model3, and a melanoma mouse model where they prevented tumor immunity4. In addition, in vitro suppression assays were performed with isolated regulatory T cells.5,6 |
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| Figure 1 |
| Isolation of CD4+CD25+ regulatory T cells from a mouse spleen cell suspension using the CD4+CD25+ Regulatory T Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ Separator, and a MiniMACS™Separator. |
| Spleen cells before separation |
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| CD4+ T cells after depletion of non-CD4+ T cells |
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| Isolated CD4+CD25+ regulatory T cells |
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| Figure 2 |
| Working scheme of the CD4+CD25+ Regulatory T Cell Isolation Kit. |
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