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MACS® Cell Separation Reagents
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CD90.1 MicroBeads
Description
CD90.1 MicroBeads, mouse and rat, are suitable for positive selection or depletion of mouse T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood.
The CD90.1 MicroBeads react with rat CD90 (Thy-1) and mouse CD90.1 (Thy1.1), a GPI-anchored conserved membrane glycoprotein. In the mouse strains AKR/J, PL, and FVB/N, CD90.1 is a pan T cell marker and can be found on thymocytes, hematopoietic stem cells in the bone marrow, intraepithelial cells (dendritic epidermal cells) in skin, and on neurons, such as retinal ganglion cells.
The antibody does not cross-react with CD90.2 (Thy1.2).
Applications
CD90.1 MicroBeads are suitable for positive selection or depletion of mouse (strains AKR/J, PL, and FVB/N) or rat T lymphocytes from single-cell suspensions of spleen preparations or peripheral blood.
Isolation of T cells for the adoptive transfer of CD90.1+ T cells into CD90.2+ inbred strains or immunodeficient mice.
Columns
For positive selection: MS, LS, and autoMACS™ Columns; for depletion: LD and autoMACS™ Columns.
 
Figure 1
CD90.1+ cells were isolated from a single-cell suspension of mouse spleen using the CD90.1 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD90.1-PE and CD45R (B220)-APC and analyzed using the MACSQuant® Analyzer.
A: Before separation
B: CD90.1cells
C: Isolated CD90.1+ cells
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CD90.1 MicroBeads, mouse and rat
- for 2×109 total cells
Download datasheet
130-094-523
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CD90.1 antibodies
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